ISIS Neutron and Muon Source Data Journal

NR study of binding of short antimicrobial alpha-helix peptides with model lipid monolayer at the air/water interface

Abstract: In this work we explore how our rationally designed peptide amphiphiles bind onto lipid monolayers using neutron reflection combined with deuterium labelling to the lipids, peptides and water. In the home work so far, we have developed lipid monolayer models mimicking mammalian and bacteria (G-, G+) outer cell membranes. To start this part of study, we propose to study how the G4 peptide bind to single and binary component monolayer models to understand how charges and saturation and membrane composition affect the amount of peptide binding and the extent of insertion. The careful use of isotopic contrasts will allow us to analyse the structures using the kenematic approach. 5 days of Surf beam time is requested to complete the tasks as proposed. This work will provide direct molecular insight of peptide selectivity to different membranes.

Principal Investigator: Professor Jian Lu
Experimenter: Mr Charles Smith
Experimenter: Mr Robert Holman
Experimenter: Dr Zongyi Li
Local Contact: Dr Mario Campana
Experimenter: Miss Daniela Ciumac
Experimenter: Dr John Webster
Experimenter: Dr Arwel Hughes

DOI: 10.5286/ISIS.E.RB1520191

ISIS Experiment Number: RB1520191

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Jian Lu et al; (2015): NR study of binding of short antimicrobial alpha-helix peptides with model lipid monolayer at the air/water interface, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1520191

Data is released under the CC-BY-4.0 license.