ISIS Neutron and Muon Source Data Journal

The structure and orientation of the Bam complex in the Gram-negative outer membrane

Abstract: The proteins of the outer membrane of Gram-negative bacteria are critical for bacterialsurvival, from providing basic physiological functions, to aiding virulence and multidrugresistance. The Bam complex has been found to be essential for folding these proteins intothe outer membrane. The process by which this is achieved is unknown. Key to thisunderstanding is the knowledge of the structure of the complex and its individualcomponents. We aim to continue our investigation of the Bam complex by studying the complete BamABCDE complex and in particular identify how the individual components are organised on the membrane surface. To do this will use neutron reflectometry and specific deuteration to probe the structure of the Bam complex in a model E.coli outer membrane and elucidate the scaffolding of this essential complex on the membrane surface for the first time.

Principal Investigator: Dr Tim Knowles
Experimenter: Dr Luke Clifton
Experimenter: Mr Lee Quill
Experimenter: Dr Mark Jeeves
Experimenter: Dr Stephen Hall
Experimenter: Mrs Pooja Sridhar

DOI: 10.5286/ISIS.E.RB1610262

ISIS Experiment Number: RB1610262

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
Select the data format above to find out more about it.

Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Dr Tim Knowles et al; (2016): The structure and orientation of the Bam complex in the Gram-negative outer membrane, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1610262

Data is released under the CC-BY-4.0 license.