ISIS Neutron and Muon Source Data Journal

Investigation of the solution structure and pore dynamics of Bacillus anthracis protective antigen stabilised in lipid nanodiscs.

Abstract: Understanding the nature of protein translocating events through bacterial pores offers an uniqueopportunity to develop novel drug delivery systems, whilst also providing invaluable informationrelating to their biology. As part of this proposal we wish to evaluate protein translocation eventsthrough lipid nanodiscs containing protective antigen (PA), a pore forming protein produced by the bacterium Bacillus anthracis. The ability of this pore to translocate large proteins is well documented, current theory states these must unfold to allow for passage through lumen, an energy intensive process. Our hypothesis is that proteins require the PA pore to expand allowing for transit. The key objective is to determine the PA pore shape, solution structure and structural alterations that occur as a function of pH using SANS, complementing our neutron reflectometry experiment at ILL (8-03-848).

Principal Investigator: Dr Paul Dyer
Experimenter: Miss Monique Pattrick
Experimenter: Miss Susan Shorter
Experimenter: Dr Beatrice Cattoz
Local Contact: Dr James Doutch
Experimenter: Dr Rob Barker

DOI: 10.5286/ISIS.E.RB1610353

ISIS Experiment Number: RB1610353

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Dr Paul Dyer et al; (2016): Investigation of the solution structure and pore dynamics of Bacillus anthracis protective antigen stabilised in lipid nanodiscs., STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1610353

Data is released under the CC-BY-4.0 license.