ISIS Neutron and Muon Source Data Journal

Lipodepsipeptides and Brown Blotch Disease in Mushrooms (i) Effect of biogenic amines

Abstract: Tolaasin is a lipodepsipeptide (lipo = hydrophobic side chain, depsipeptide is an amino acid ring closed in a particular way) produced by strains of Pseudomonas tolaasii, and it causes Brown Blotch disease in mushrooms. This is a major problem in commercial mushroom growing. Tolaasin is detected by the formation of a "white line" when it comes into contact with the lipodepsipeptides, WLIP (White Line Inducing Peptide) created by Pseudomonas reactans. WLIP is evidently antagonistic to tolaasin but the mechanism is not understood. White line formation is enhanced by the biogenic amine, octopamine. Preliminary reflection experiments confirm a strong specific interaction of octopamine and WLIP. This seems to require an unfolding of the known solid state structure of WLIP and we propose to determine the layer structure using reflectometry and part deuterated WLIP.

Principal Investigator: Professor Gail Preston
Experimenter: Dr Souvik Kusari
Experimenter: Dr Peixun Li
Experimenter: Professor Jeffery Penfold
Experimenter: Dr Bob Thomas
Experimenter: Mr Marcel Bach Pages
Local Contact: Dr Rebecca Welbourn

DOI: 10.5286/ISIS.E.RB1610101

ISIS Experiment Number: RB1610101

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
Select the data format above to find out more about it.

Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Gail Preston et al; (2016): Lipodepsipeptides and Brown Blotch Disease in Mushrooms (i) Effect of biogenic amines, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1610101

Data is released under the CC-BY-4.0 license.