ISIS Neutron and Muon Source Data Journal

Dynamics of amyloid protein fibril elongation using isotope-labelled SANS

Abstract: This proposal combines optical spectroscopy with neutron scattering using novel labelling strategies to investigate ?amyloid? protein fibrils. Variations in packing and in the dynamics of elongation and breakage underpin phenomena ranging from non-genetic heredity in yeast, to diseases such as Alzheimer?s, BSE and CJD. Most solution methods to monitor growth dynamics only give information on the overall % of material transformed from non-fibrillar to fibrillar forms. They cannot determine length distribution or linear growth rates, but instead convolute the two; in other words, they cannot distinguish a few long fibrils each growing quickly, from many short fibrils each growing more slowly. We will determine both fibril lengths and linear growth rates using SANS and insitu optical spectroscopy.

Principal Investigator: Dr Adam Squires
Experimenter: Miss Eleonore Mason
Local Contact: Dr Richard Heenan
Experimenter: Dr Ann Terry
Local Contact: Dr Sarah Rogers
Local Contact: Dr James Doutch
Experimenter: Mr Ben Eves
Experimenter: Dr Tim Snow

DOI: 10.5286/ISIS.E.RB1520491

ISIS Experiment Number: RB1520491

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
Select the data format above to find out more about it.

Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Dr Adam Squires et al; (2016): Dynamics of amyloid protein fibril elongation using isotope-labelled SANS, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1520491

Data is released under the CC-BY-4.0 license.