ISIS Neutron and Muon Source Data Journal

Neutron Reflectivity Study of Protein Adsorption onto Cell Membrane-inspired Anti-Fouling Phospholipid Polymer Brush Surface

Abstract: We grafted 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer brush from the silicon wafer surface through Cu (0) mediated controlled radical polymerization (CuCRP) to mimic cell membrance. Excellent control over graft density and film thickness was acheived by reaction condition variation. An optimal thickness of PMPC for protein resistance was estabilished. The adsorption then was found to increase together with the PMPC thickness. This can be either attributed to an increased surface adsorption or to protein penetration within the structure.In order to progress our understanding of the system further, we need to determine the protein distribution across the PMPC layer as a function of both protein concentration and graft density. Because of the advantage offered by contrast variation and the Ångstrom resolution, neutron reflectivity is the ideal technique to study these systems.

Principal Investigator: Professor Emil Chibowski
Experimenter: Dr Mario Campana
Experimenter: Dr Aleksandra Szczes
Experimenter: Miss Yingdi Yan

DOI: 10.5286/ISIS.E.RB1800016

ISIS Experiment Number: RB1800016

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Emil Chibowski et al; (2017): Neutron Reflectivity Study of Protein Adsorption onto Cell Membrane-inspired Anti-Fouling Phospholipid Polymer Brush Surface, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1800016

Data is released under the CC-BY-4.0 license.