ISIS Neutron and Muon Source Data Journal

Repeat of “Assembling floating, very sparsely tethered, protein-lipid bilayers”.

Abstract: This was granted 2 days but problems with our samples meant that no data was got. After successful practice on Biacore offline, failure was due to using a new (faulty) batch of thiolipid /linker. By using a single batch for both we will avoid this problem in future. Tethered floating lipid bilayers are useful models of biological membranes in which a phospholipid bilayer is tethered to a solid surface by a molecular linker. They promise an additional method to help us build accurate models of all types of membranes. Our previous POLREF experiments showed that we can assemble lipid only bilayers at very low tether densities which is important to minimise their effect on the membrane. Here we wish to show that we can insert and characterise proteins within the lipid bilayer which is another important step in creating useful models of living membranes in neutron tractable sample environmen

Principal Investigator: Professor Jeremy Lakey [Deactivated] [Deactivated]
Experimenter: Dr Nicolò Paracini
Experimenter: Dr Luke Clifton
Local Contact: Dr Andrew Caruana
Experimenter: Dr Martynas Gavutis

DOI: 10.5286/ISIS.E.RB1720245

ISIS Experiment Number: RB1720245

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Jeremy Lakey [Deactivated] [Deactivated] et al; (2017): Repeat of “Assembling floating, very sparsely tethered, protein-lipid bilayers”. , STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1720245

Data is released under the CC-BY-4.0 license.