ISIS Neutron and Muon Source Data Journal

The Interaction of Small Cationic Amphiphillic Antimicrobial Proteins with Free Floating Model of the Gram Negative Bacterial Outer membrane

Abstract: Protein-membranes interactions are fundamentally important to biological function. We have developed advanced models of the Gram negative bacterial outer membrane and have examined the interaction of a1-Purothionin (Pth) with these models under different conditions to structurally probe the mode in which cationic amphiphilic proteins such as thionins and defensins interact with bacterial membranes to exert their antimicrobial activity. Results to date reveal that the interaction of a1-Pth with the bilayer tails appears to be mediated by bilayer defects. However, the lack of a mobile inner bilayer leaflet in the silicon supported bilayers studied maybe hampering the proteins interaction. Therefore here, we plan to examine the interaction of a1-Pth with free floating asymmetric models of the Gram negative bacterial outer membrane to gain further structural insights into h

Principal Investigator: Dr Luke Clifton
Experimenter: Dr Martynas Gavutis
Experimenter: Professor Jeremy Lakey [Deactivated] [Deactivated]
Experimenter: Professor Rebecca Green
Local Contact: Dr Andrew Caruana
Experimenter: Dr Maxmilian Skoda
Experimenter: Professor Richard Frazier

DOI: 10.5286/ISIS.E.RB1710470

ISIS Experiment Number: RB1710470

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Dr Luke Clifton et al; (2017): The Interaction of Small Cationic Amphiphillic Antimicrobial Proteins with Free Floating Model of the Gram Negative Bacterial Outer membrane, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1710470

Data is released under the CC-BY-4.0 license.