ISIS Neutron and Muon Source Data Journal

Introducing the O-antigen into Bacterial Outer Membrane Models

Abstract: The Gram negative bacterial outer membrane protects the bacterium from external toxins such as antibiotics. This is partially responsible for Gram negative bacteria becoming the greatest emerging source of antibiotic resistance. We are developing models of these bacterial outer membranes and have successfully recreated the characteristic lipid asymmetry (with lipopolysaccharide (LPS) on the outer leaflet and phospholipids on the inner) and also the water filled periplasm. In order to fully recreate the membranes we will need to incorporate more complex LPS versions to mimic pathogenic bacteria. We will incorporate LPS containing one or two O antigens into the final model at different densities to determine who much it increases the bilayer thickness. In future experimental programmes we aim to increase the LPS length to investigate how very long LPS behave.

Principal Investigator: Professor Jeremy Lakey [Deactivated] [Deactivated]
Experimenter: Dr Luke Clifton
Experimenter: Dr Nicolò Paracini
Experimenter: Dr Maxmilian Skoda

DOI: 10.5286/ISIS.E.RB1700060

ISIS Experiment Number: RB1700060

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Jeremy Lakey [Deactivated] [Deactivated] et al; (2017): Introducing the O-antigen into Bacterial Outer Membrane Models, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1700060

Data is released under the CC-BY-4.0 license.