ISIS Neutron and Muon Source Data Journal

Assembling floating, very sparsely tethered, protein-lipid bilayers.

Abstract: We have recently used Polref in to measure the assembly of tethered floating lipid bilayers. These are useful models of biological membranes in which a phospholipid bilayer is tethered to a solid surface by a molecular linker. The length of this linker can control the width of the water gap between the surface and the floating bilayer. Since they are easy to make and characterise , they promise an additional method to help us build accurate models of bacterial outer membranes. Here the water gap represents an important cellular compartment called the periplasm. Our previous POLREF experiments showed that we can assemble bilayers at very low tether densities which is important to minimise their effect on the membrane. Here we wish to show that we can insert and characterise proteins within the lipid bilayer which is another important step in creating useful models of living membranes

Principal Investigator: Professor Jeremy Lakey [Deactivated] [Deactivated]
Experimenter: Dr Martynas Gavutis
Experimenter: Dr Nicolò Paracini
Experimenter: Dr Luke Clifton
Local Contact: Dr Christy Kinane

DOI: 10.5286/ISIS.E.RB1710218

ISIS Experiment Number: RB1710218

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
Select the data format above to find out more about it.

Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Jeremy Lakey [Deactivated] [Deactivated] et al; (2017): Assembling floating, very sparsely tethered, protein-lipid bilayers. , STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1710218

Data is released under the CC-BY-4.0 license.