ISIS Neutron and Muon Source Data Journal
This is a page describing data taken during an experiment at the ISIS Neutron and Muon Source. Information about the ISIS Neutron and Muon Source can be found at https://www.isis.stfc.ac.uk.
Adsorption of bioengineered antibodies at the oil/water interface
Abstract: Following our previous study of adsorption of Fc, Fab and the constituent mAb COE-3, we continue to explore how two bioengineered mAbs adsorb at the oil/water interface by neutron reflection. Inter is preferred because we have built technical capability on this instrument. COE-7 and COE-8 have enlarged Fab and Fc domains and reduced globular stability. Our specific aims are to (a) determine the actual thickness and volume fraction of and (b) the effect of pH on the interfacial distribution. The results will enable us to compare their extent of distributions into the oil region with other globular proteins at the interface. We can then answer if all the globular proteins unfold and produce characteristic segment distributions into the oil region.
Principal Investigator: Professor Jian Lu
Local Contact: Dr Maxmilian Skoda
Experimenter: Dr Mario Campana
Experimenter: Dr Zongyi Li
Experimenter: Mr Sean Ruane
Experimenter: Dr John Webster
Experimenter: Mr Ruiheng Li
Experimenter: Dr Xuzhi Hu
DOI: 10.5286/ISIS.E.RB1710294
ISIS Experiment Number: RB1710294
| Part DOI | Instrument | Public release date | Download Link |
|---|---|---|---|
| 10.5286/ISIS.E.84939600 | INTER | 21 March 2020 | Download |
Publisher: STFC ISIS Neutron and Muon Source
Data format: RAW/Nexus
Select the data format above to find
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Data Citation
The recommended format for citing this dataset in a research
publication is as:
[author], [date], [title], [publisher],
[doi]
For Example:
Professor Jian Lu et al; (2017): Adsorption of bioengineered antibodies at the oil/water interface, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1710294
Data is released under the CC-BY-4.0 license.
