ISIS Neutron and Muon Source Data Journal

A biophysical approach of studying the molecular interactions of a novel antibiotic with the Gram negative bacterial cell envelope.

Abstract: Gram negative (-ve) bacteria possess numerous defenses against antibiotics, not the least of them being the intrinsic permeability barrier afforded by their outer membrane (OM) and various efflux mechanisms which pump out drugs. A novel compound was synthesized at the KCL which shows promising activity against Gram (-ves). However, there was a noticeable effect of drug efflux resulting in reduced efficacy. This project investigates novel formulations to increase OM permeability to the antibiotic. Drug/enhancer complexes were made, which pass into the cell and dissociate thereby circumventing intrinsic resistance and membrane-associated efflux mechanisms. Neutron reflectivity will offer a greater insight into the intimate molecular drug (pure and formulated)-membrane interactions in order to understand how drug uptake may be improved for achieving optimal antimicrobial activity

Principal Investigator: Dr Cecile Dreiss
Experimenter: Dr Olivia Pabois
Local Contact: Dr Maxmilian Skoda
Experimenter: Miss Kalliopi-Kelli Vandera
Experimenter: Dr Richard Harvey

DOI: 10.5286/ISIS.E.RB1710319

ISIS Experiment Number: RB1710319

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Dr Cecile Dreiss et al; (2017): A biophysical approach of studying the molecular interactions of a novel antibiotic with the Gram negative bacterial cell envelope., STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1710319

Data is released under the CC-BY-4.0 license.