ISIS Neutron and Muon Source Data Journal

Antibiotic Interactions with models of the Gram negative bacterial outer membrane : Physiological Temperature Polymyxin B Titration

Abstract: Gram-negative bacteria, which cause a variety of diseases including meningitis, plague, sepsis and food poisoning, are becoming increasingly antibiotic resistant. One reason is their robust outer membrane (OM) which can resist antibiotic penetration. We have been combining our recently developed OM model with neutron science to measure the penetration of an important antibiotic, polymyxin (PMB), into the OM. These have shown the importance of the critical melting of the OM at body temperature in allowing PMB to work. These have been supported by in vivo data on live bacteria. We need to measure how strongly the PMB binds the OM at physiological temperature compared to the lower temperatures already measured. This titration experiment using increasing PMB concentrations at 37C will provide critical final data and allow us to publish in a high impact journal.

Principal Investigator: Professor Jeremy Lakey [Deactivated] [Deactivated]
Local Contact: Dr Christy Kinane
Experimenter: Dr Nicolò Paracini
Experimenter: Dr Luke Clifton
Experimenter: Dr Maxmilian Skoda

DOI: 10.5286/ISIS.E.RB1700083

ISIS Experiment Number: RB1700083

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Jeremy Lakey [Deactivated] [Deactivated] et al; (2017): Antibiotic Interactions with models of the Gram negative bacterial outer membrane : Physiological Temperature Polymyxin B Titration , STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1700083

Data is released under the CC-BY-4.0 license.