ISIS Neutron and Muon Source Data Journal
This is a page describing data taken during an experiment at the ISIS Neutron and Muon Source. Information about the ISIS Neutron and Muon Source can be found at https://www.isis.stfc.ac.uk.
Photocrystallography in retinal
Abstract: Retinal plays a key role in visual perception. In the ground state it occurs in the cis form with a bent side chain, whilst after light exposure it assumes the trans form with the methyl groups on one side and the side chain straight. Laboratory X-ray data collections are a bit challenging due to radiation damage. More than half of the atoms are hydrogen. We have established a protocol to load samples cold into the top loading CCR and found that crystals remain stable under He atmosphere at room temperature. This opens the possibility to do a multi temperature data collection using neutrons and study the protons in both forms. We plan to collect data at 4.5K, 50K, 100K, 200K and 300K and then monitor the cis to trans transformation at 100K under light exposure on the beamline. We ask for 6 days of beamtime on SXD equipped with a top-loading CCR. Light exposure will be done using diodes.
Principal Investigator: Dr Matthias Gutmann
Experimenter: Dr Hazel Sparkes
DOI: 10.5286/ISIS.E.RB1710030
ISIS Experiment Number: RB1710030
| Part DOI | Instrument | Public release date | Download Link |
|---|---|---|---|
| 10.5286/ISIS.E.84782431 | SXD | 24 October 2020 | Download |
Publisher: STFC ISIS Neutron and Muon Source
Data format: RAW/Nexus
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Data Citation
The recommended format for citing this dataset in a research
publication is as:
[author], [date], [title], [publisher],
[doi]
For Example:
Dr Matthias Gutmann et al; (2017): Photocrystallography in retinal, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1710030
Data is released under the CC-BY-4.0 license.
