ISIS Neutron and Muon Source Data Journal

Deposition of lipid bilayer through peptide-discs

Abstract: We are developing a new protocol for the preparation of lipid bilayers suitable to host membrane proteins with control on their orientation. Our proposed strategy is based on anchoring the membrane proteins re-constituted in nanodiscs to the support surface. Peptide-discs are nanodiscs where a lipid bilayer is surrounded by a belt of self-assembled peptides, which can easily be removed by buffer rinsing. As first step toward the development of this new protocol first of all we propose to understand the fundamentals of peptide-discs mediated lipid bilayer formation. During a preliminary test, we have verified by NR that peptide-discs can be used to form a POPC bilayer. Here we propose NR measurement to assess the reproducibility of this system and to test the effect of different lipid composition on the peptide-disc deposition.

Principal Investigator: Dr Alessandra Luchini
Experimenter: Dr Raul Araya Secchi
Experimenter: Dr Mario Campana

DOI: 10.5286/ISIS.E.RB1820625

ISIS Experiment Number: RB1820625

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Dr Alessandra Luchini et al; (2018): Deposition of lipid bilayer through peptide-discs, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1820625

Data is released under the CC-BY-4.0 license.