ISIS Neutron and Muon Source Data Journal

Dynamics of the myoglobin-sucrose-water system for understanding the role of cryoprotectants

Abstract: We intend to study the dynamics of the myoglobin-sucrose-water system, with the aim to understand why trehalose exhibits better cryopreservation and stabilization properties than sucrose. We have already performed QENS studies on the corresponding trehalose system (RB 1600024), as well as NIMROD experiments on both systems (RB 1520094 and RB 1700070), with complementary EPSR modelling and MD simulations to guide us in the interpretations of the NIMROD and IRIS data, respectively. Here, we particularly aim to elucidate whether the differences between trehalose and sucrose can be explained by a smaller reduction of the protein dynamics in the case of sucrose. This experiment will furthermore be used as a “student study” in a neutron spectroscopy course within the Swedish national graduate school in neutron scattering (SwedNESS) and, therefore, would need to be scheduled on 26 Nov 2018.

Principal Investigator: Professor Jan Swenson
Experimenter: Miss Filippa Lundin
Experimenter: Ms Stefanie Siebeneichler
Experimenter: Miss Karolina Mothander
Experimenter: Dr Gustav Ek
Experimenter: Miss Judith Ingrid Marie Lycksell
Experimenter: Mr Paulo Henrique Barros Brant Carvalho
Experimenter: Mr Christoffer Olsson
Experimenter: Dr Franz Demmel

DOI: 10.5286/ISIS.E.RB1820165

ISIS Experiment Number: RB1820165

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Jan Swenson et al; (2018): Dynamics of the myoglobin-sucrose-water system for understanding the role of cryoprotectants, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1820165

Data is released under the CC-BY-4.0 license.