ISIS Neutron and Muon Source Data Journal

Zwitterionic Polymers for Lipid Nanodisc Stabilisation

Abstract: Polymer stabilized lipid nanodiscs offer enormous potential as tools for enabling membrane protein structural studies & biophysics. Previously we studied discs formed using a poly(styrene-alt-maleic acid) (PSMA) copolymer, and also with a commercial poly(styrene-alt-maleimide). However these polymers can be used only over limited pH ranges, as they precipitate when their charge is neutralised. To overcome this we have devised a simple modification of the hydrolysis procedure which produces a zwitterionic version of the copolymer from the same initial poly(styrene-alt-maleic anhydride). The modification can be done to either the widely used commercial polymers, or our controlled Mw copolymers from RAFT polymerisations. Here we wish to measure particles formed using zwitterionic copolymers with DMPC to confirm that they are nanodiscs and to compare to those formed using the acid copolymers

Principal Investigator: Professor Karen Edler
Experimenter: Mr George Neville
Experimenter: Dr James Doutch
Experimenter: Mr Jake Hooton
Experimenter: Ms Kerrie Morrison
Experimenter: Dr Andrew McCluskey
Experimenter: Dr Luke Clifton
Experimenter: Ms Ria Atri
Experimenter: Dr Marcelo da Silva

DOI: 10.5286/ISIS.E.RB1820480

ISIS Experiment Number: RB1820480

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
Select the data format above to find out more about it.

Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Karen Edler et al; (2018): Zwitterionic Polymers for Lipid Nanodisc Stabilisation, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1820480

Data is released under the CC-BY-4.0 license.