ISIS Neutron and Muon Source Data Journal

Elucidating the Membrane Interaction Mechanism of Chloride Intracellular Channel Proteins

Abstract: Chloride Intracellular Channels (CLICs) are a family of 6 proteins that have in common a C-terminal CLIC module and chloride-selective ion channel activity. CLIC1 is a 241 amino-acid protein involved in the regulation of the cell cycle, in particular it is overexpressed in a number of cancer cells. However, to date little information exists as to its structure and the factors that influence its insertion into membranes. Here we propose to study non-oxidised CLIC1 at pH 5.5 and 7.4 against two membrane compositions (i) PC:PE:Chol and (ii) PC:Chol. This will be used in combination with a broad range of ongoing biophysical characterisation to establish the mechanism of CLIC1 chloride channel formation, as well as the factors influencing it. This will be an initial step to understand how CLIC1 functions in tumour cells and will allow specific treatments to be designed to interrupt it.

Principal Investigator: Dr Jose Luis Ortega Roldan
Local Contact: Dr Mario Campana
Experimenter: Dr Rob Barker
Experimenter: Miss Irantzu Yoldi
Experimenter: Miss Alex Hendry

DOI: 10.5286/ISIS.E.RB1820565

ISIS Experiment Number: RB1820565

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Dr Jose Luis Ortega Roldan et al; (2018): Elucidating the Membrane Interaction Mechanism of Chloride Intracellular Channel Proteins, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1820565

Data is released under the CC-BY-4.0 license.