ISIS Neutron and Muon Source Data Journal

Probing the structure/function relationship of NhaA protein incorporated into tethered lipid bilayers

Abstract: We propose to perform combined measurements of neutron reflectometry (NR) and impedance spectroscopy (IS) to investigate the structure/function relationship of tethered bilayer lipid membranes (tBLM) on gold substrates with incorporated membrane transport proteins. This biomimetic membrane system is the core of the bio-fuel cell that we are developing to create energy in a bio-inspired way. Previous experiments have shown that lipid bilayers with a high fraction of cardiolipin show interesting properties for electrochemical application and that the incorporation of the sodium proton antiporter protein, NhaA, is possible. We want to extend the study to different lipid compositions, protein incorporation methods and tethered lipid molecular composition to optimize the membrane-protein system in terms of stability, efficiency in protein incorporation and electrochemical properties.

Principal Investigator: Dr Marco Maccarini
Experimenter: Dr jean-pierre Alcaraz
Experimenter: Mr Sebastian Köhler
Experimenter: Dr Giovanna Fragneto
Experimenter: Professor Donald Martin
Local Contact: Dr Mario Campana
Experimenter: Miss Valeria Italia

DOI: 10.5286/ISIS.E.RB1810868

ISIS Experiment Number: RB1810868

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Dr Marco Maccarini et al; (2018): Probing the structure/function relationship of NhaA protein incorporated into tethered lipid bilayers , STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1810868

Data is released under the CC-BY-4.0 license.