ISIS Neutron and Muon Source Data Journal

Copy of: Dynamics of amyloid protein fibril elongation using isotope-labelled SANS

Abstract: This proposal combines small-angle neutron scattering with novel deuteration strategies to investigate amyloid protein fibrils. Variations in dynamics of elongation and breakage underpin phenomena ranging from non-genetic heredity in yeast, to diseases such as Alzheimers, BSE and CJD. However, solution methods to monitor growth dynamics only give information on the overall % of material transformed from non-fibrillar to fibrillar forms. They cannot determine elongation rate directly, instead convoluting it with length distribution; and so cannot distinguish a few long fibrils growing quickly from many short fibrils growing slowly. We have developed a SANS approach to measure elongation rate, with isotope labelled ends growing on contrast-matched "seed" fibrils, demonstrated by proof -of-concept data on SANS2D in 2016. This proposal will enable us to finish the study, to allow publication

Principal Investigator: Dr Adam Squires
Experimenter: Mr Ben Eves
Experimenter: Dr James Doutch
Experimenter: Dr Michael Haertlein
Experimenter: Dr Trevor Forsyth
Experimenter: Dr Ann Terry
Experimenter: Mr Han Yin

DOI: 10.5286/ISIS.E.RB1810401

ISIS Experiment Number: RB1810401

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Dr Adam Squires et al; (2018): Copy of: Dynamics of amyloid protein fibril elongation using isotope-labelled SANS, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1810401

Data is released under the CC-BY-4.0 license.