ISIS Neutron and Muon Source Data Journal

Dynamic Adsorption of the Fusion Protein HSA-NEP at the Silica-Water Interface

Abstract: HSA-NEP is a fusion protein with potential uses in treating Alzheimer’s disease. It consists of Neprilysin, a peptide degrading enzyme, linked to HSA, the most common protein in blood plasma. Fusion proteins such as HSA-NEP are often stored in glass (silica) containers, to which they adsorb, often causing denaturation of the protein. We have investigated dynamic adsorption of HSA-NEP to the silica-water interface using spectroscopic ellipsometry and FTIR to determine the adsorbed amount and secondary structure changes, but this does not give a true physical structural picture of the protein adsorbed. Dynamic neutron reflection measurements will be unique to unravel how interfacial structure of HSA-NEP changes as a function of time, pH and concentration. As the pIs for HSA and NEP are different, it is interesting to explore how they change in the adsorbed layer with pH.

Principal Investigator: Professor Jian Lu
Experimenter: Dr Mingrui Liao
Experimenter: Mr Sean Ruane
Experimenter: Mr Zhe Li
Experimenter: Dr Xuzhi Hu
Experimenter: Dr Chris van der Walle
Local Contact: Dr Jos Cooper
Experimenter: Mr Peter Hollowell

DOI: 10.5286/ISIS.E.RB1820134

ISIS Experiment Number: RB1820134

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
Select the data format above to find out more about it.

Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Jian Lu et al; (2018): Dynamic Adsorption of the Fusion Protein HSA-NEP at the Silica-Water Interface, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1820134

Data is released under the CC-BY-4.0 license.