ISIS Neutron and Muon Source Data Journal

Using SANS2D to determine the size of MscL clusters in proteoliposomes

Abstract: In bacteria, mechanosensitive ion channels such as MscL protect cells against osmotic shock. Although crucial for protecting cells, as with all ion channels, they have the potential to act as an Achilles heel, by providing a target for antimicrobial treatments. It is thought that these work as pressure release valves and respond to an increase in membrane tension by opening. Previous measurements suggested that these ion channels assemble into clusters of 100's of channels. Having a few channels in a cluster enables the sensitivity of the valve to be tuned having 100's of proteins in a cluster does not seem to make biological sense. Our SAXS measurements suggest that by expressing the protein direct into the proteoliposomes, we have created a system that is more representative of that found in bacterial membranes, so we should be able to get an accurate picture of the cluster size.

Principal Investigator: Dr Simon Titmuss
Experimenter: Dr Maxmilian Skoda
Experimenter: Dr Sophie Ayscough
Experimenter: Dr James Doutch
Experimenter: Miss Rashmi Seneviratne

DOI: 10.5286/ISIS.E.RB1820511

ISIS Experiment Number: RB1820511

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Dr Simon Titmuss et al; (2018): Using SANS2D to determine the size of MscL clusters in proteoliposomes, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1820511

Data is released under the CC-BY-4.0 license.