ISIS Neutron and Muon Source Data Journal

Effect of squalene on membrane structure and Amphotericin B resistance in Candida glabrata.

Abstract: The yeast Candida glabrata is becoming an increasing cause of infections. We have developed RNA interference tools that allow us to study the virulence and resistance mechanisms in C. glabrata by upregulating/downregulating specific genes, and we determine the corresponding changes in its cell membrane lipid and sterol composition. We recently observed an accumulation of squalene in a C. glabrata strain with resistance to the antifungal drug Amphotericin B (AmB), which acts by binding ergosterol. Our previous neutron reflectivity measurements have shown that reconstituted C. glabrata membranes have different binding affinity for AmB depending on the sterol composition. As squalene may contribute to altered susceptibility to AmB, we propose in this experiment to investigate the effect of squalene on the structure of C. glabrata and POPC lipid bilayers, and their response to AmB.

Principal Investigator: Dr Wolfgang Knecht
Experimenter: Mr Juan Manuel Orozco Rodriguez
Local Contact: Dr Maxmilian Skoda
Experimenter: Dr Hanna Wacklin-Knecht
Experimenter: Mr Robin Delhom

DOI: 10.5286/ISIS.E.RB1820286

ISIS Experiment Number: RB1820286

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Dr Wolfgang Knecht et al; (2018): Effect of squalene on membrane structure and Amphotericin B resistance in Candida glabrata., STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1820286

Data is released under the CC-BY-4.0 license.