ISIS Neutron and Muon Source Data Journal

Introducing the O-antigen in Bacterial Outer Membrane in vitro Models Pt. III The effect upon antibiotic sensitivity

Abstract: The Gram negative bacterial outer membrane protects the bacterium from external toxins such as antibiotics. This is partially responsible for Gram negative bacteria becoming the fastest emerging source of antibiotic resistance. We are developing models of these bacterial outer membranes and have successfully recreated the characteristic lipid asymmetry (with lipopolysaccharide (LPS) on the outer leaflet and phospholipids on the inner). In order to fully recreate the membranes we will need to incorporate more complex LPS versions to mimic pathogenic bacteria. Pathogenic bacteria contain a proportion of longer LPS molecules which are related to their virulence. Recently we have incorporated LPS containing one or two O antigens into the model and now wish to understand the role of the O antigen in resistance towards outer membrane disrupting antibiotics such as polymyxin B.

Principal Investigator: Professor Jeremy Lakey [Deactivated] [Deactivated]
Experimenter: Dr Luke Clifton
Experimenter: Dr Nicolò Paracini
Experimenter: Dr Maxmilian Skoda

DOI: 10.5286/ISIS.E.RB1820430

ISIS Experiment Number: RB1820430

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Jeremy Lakey [Deactivated] [Deactivated] et al; (2018): Introducing the O-antigen in Bacterial Outer Membrane in vitro Models Pt. III The effect upon antibiotic sensitivity, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1820430

Data is released under the CC-BY-4.0 license.