ISIS Neutron and Muon Source Data Journal

Adsorption of Bispecific Monoclonal Antibodies with Extended Domains at the Oil/Water Interface

Abstract: Following our previous study of interfacial adsorption of the basic version mAb COE-3, we wish to continue this work by exploring how two modified mAbs adsorb at the same oil/water interface by neutron reflection. Inter is preferred because we have built technical capability on this instrument. COE-7 and COE-8 have enlarged Fab and Fc domains and reduced globular stability. Our specific aims are to (a) determine the actual thickness and volume fraction of them and (b) examine the effect of pH on their interfacial distribution. The results will enable us to compare their extent of distributions into the oil region with other globular proteins at the interface. We can then answer if all the globular proteins unfold and produce characteristic segment distributions into the oil region.

Principal Investigator: Professor Jian Lu
Local Contact: Dr Maxmilian Skoda
Experimenter: Dr Mario Campana
Experimenter: Dr Zongyi Li
Experimenter: Mr Sean Ruane
Experimenter: Mr Ruiheng Li
Experimenter: Mr Peter Hollowell
Experimenter: Dr Mingrui Liao

DOI: 10.5286/ISIS.E.RB1820210

ISIS Experiment Number: RB1820210

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
Select the data format above to find out more about it.

Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Jian Lu et al; (2018): Adsorption of Bispecific Monoclonal Antibodies with Extended Domains at the Oil/Water Interface, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1820210

Data is released under the CC-BY-4.0 license.