ISIS Neutron and Muon Source Data Journal

Lipid exchange between polymer stabilised nanodiscs and biomimetic bilayers at the solid-liquid interface

Abstract: Polymer-stabilised lipid nanodiscs are discoidal structures composed on a central core of lipid bilayer encapsulated and stabilised in aqueous solution by amphipathic copolymers. Nanodiscs are emerging as powerful tools in the structural and functional characterisation of integral membrane proteins with potential application as a drug delivery platform. Recent studies have provided evidence of lipid exchange between nanodiscs in solution and lipid membranes at interfaces. We aim to complete our current study investigating the kinetics of lipid exchange by structurally characterising the interaction between nanodiscs and interfacial membranes by using specific deuteration and neutron reflectometry to uncover the mechanism behind this process. This will provide an understanding of the factors influencing lipid exchange enabling a more widespread use of nanodiscs in membrane research.

Principal Investigator: Dr Thomas Arnold
Experimenter: Ms Kerrie Morrison
Experimenter: Dr Tim Knowles
Experimenter: Dr Stephen Hall
Local Contact: Dr Christy Kinane
Experimenter: Dr Luke Clifton
Experimenter: Professor Karen Edler

DOI: 10.5286/ISIS.E.RB1900009

ISIS Experiment Number: RB1900009

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Dr Thomas Arnold et al; (2018): Lipid exchange between polymer stabilised nanodiscs and biomimetic bilayers at the solid-liquid interface, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1900009

Data is released under the CC-BY-4.0 license.