ISIS Neutron and Muon Source Data Journal

DNA unwinding mechanism of the archaeal helicase PabMCM in its octameric configuration

Abstract: The process of cell division requires precise duplication of the genetic material. The separation of the two strands of DNA and the progression of the replisome are driven by replicative helicases called Mini-chromosome maintenance (MCM) proteins. MCMs unwind genomic dsDNA to expose ssDNA template during the S-phase of the cell cycle. We have recently solved the first full-length octameric structure of the Pyrococcus abyssi MCM (PabMCM) by electron microscopy and SAXS. PabMCM can be found as single or double octameric ring assemblies in absence of DNA; however, bound to dsDNA, PabMCM is found exclusively in single ring assemblies bound to the DNA substrate. We now plan to use small angle neutron scattering to solve the structure of MCM/DNA complexes with contrast matching experiments. These are unique experiments that allow to discriminate the protein and DNA component in a full complex.

Principal Investigator: Dr Laura Spagnolo
Experimenter: Dr Luke Clifton
Experimenter: Dr James Doutch
Experimenter: Miss Silvia Visentin

DOI: 10.5286/ISIS.E.RB1720488

ISIS Experiment Number: RB1720488

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Dr Laura Spagnolo et al; (2017): DNA unwinding mechanism of the archaeal helicase PabMCM in its octameric configuration, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1720488

Data is released under the CC-BY-4.0 license.