ISIS Neutron and Muon Source Data Journal

Decoupling effect between protein and water

Abstract: Water plays a critical role in life. A hydration level, h ~ 0.2 g water/g protein, is found to be the minimum for the dynamical transition in protein at Td ~ 200 K. This temperature is universal for proteins and ascribed to the surface hydration water. However, our OSIRIS neutron data on perdeuterated cytochrome P450 (CYP) and its hydrogenated counterpart showed that the Td of hydration water shifts significantly to higher values when decreasing the instrument resolution, while the Td for the protein remains essentially unchanged. OSIRIS afforded us two resolutions (25.4 and 99 ueV) which we added to data from HFBS at NIST at 1ueV resolution. These results suggests that the dynamical transition might have a different physical origin from that of its surface water. To further confirm these findings, we want to try a structurally and functionally different protein, GFP, at OSIRIS.

Principal Investigator: Professor liang hong
Experimenter: Dr Victoria Garcia Sakai
Experimenter: Mr Zhuo Liu
Experimenter: Dr Chenxing Yang

DOI: 10.5286/ISIS.E.RB1800112

ISIS Experiment Number: RB1800112

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor liang hong et al; (2018): Decoupling effect between protein and water, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1800112

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