ISIS Neutron and Muon Source Data Journal

Protein interactions at solid-liquid interfaces: The effect of affinity columns on protein structure

Abstract: Biopharmaceuticals are manufactured using chromatographic procedures including adsorption and desorption processes at the solid-liquid interface. These events take place in porous particles packed within a column. The separation is carried out under plug flow conditions, which allows the high surface area within the particles to be utilised. The traditional indirect method to measure intra-particle pore structure lacks the resolution to identify how proteins bind to these complex structures. In this application, we wish to extend initial proof of principal work which has demonstrated the use of SANS, to uncover the heterogeneous pore distribution and the sequence in which they are occupied during a chromatographic process. Our experimental design will enable us to model this and to measure the resin structure before and post adsorption with the therapeutic antibody.

Principal Investigator: Professor Daniel Bracewell
Experimenter: Professor Christopher Roberts
Experimenter: Dr James Doutch
Experimenter: Miss Maria Papachristodoulou
Experimenter: Dr Paul Butler
Experimenter: Dr Luke Clifton
Experimenter: Miss Hoi Sang Beatrice Leung

DOI: 10.5286/ISIS.E.RB1810228

ISIS Experiment Number: RB1810228

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Daniel Bracewell et al; (2018): Protein interactions at solid-liquid interfaces: The effect of affinity columns on protein structure, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1810228

Data is released under the CC-BY-4.0 license.