ISIS Neutron and Muon Source Data Journal

INTER as a new Tool to Follow the Dynamic Adsorption of Antibody and Non-ionic Surfactant

Abstract: Following our very recent work on the dynamic co-adsorption of our control antibody COE-3 and nonionic surfactant PS80-7EO from the mixed solution, We request 3 days of Inter beam time to continue this exciting study by completing parallel runs using deuterated surfactant under solvent contrasts to match out surfactant and antibody separately. In addition, we will carry out similar Inter measurements under sequential adsorption starting from COE-3 followed by surfactant binding. Given our previous work has already revealed the same adsorbed amount and layer structure from the two modes of adsorption under equilibration, our goal here is to unravel the detailed dynamic processes to help understand how mAb deformation or unfolding during interfacial adsorption could induce surfactant binding, resulting in well-organised surfactant bilayer on top of the mAb monolayer...

Principal Investigator: Professor Jian Lu
Experimenter: Mr Ruiheng Li
Experimenter: Mr Sean Ruane
Experimenter: Mr Haoning Gong
Experimenter: Dr Zongyi Li
Experimenter: Dr Lisa Pan
Experimenter: Dr Chris van der Walle
Local Contact: Dr Nina-Juliane Steinke
Experimenter: Mr Robert Holman

DOI: 10.5286/ISIS.E.RB1810237

ISIS Experiment Number: RB1810237

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Jian Lu et al; (2018): INTER as a new Tool to Follow the Dynamic Adsorption of Antibody and Non-ionic Surfactant, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1810237

Data is released under the CC-BY-4.0 license.