ISIS Neutron and Muon Source Data Journal

This is a page describing data taken during an experiment at the ISIS Neutron and Muon Source. Information about the ISIS Neutron and Muon Source can be found at https://www.isis.stfc.ac.uk.


Testing protein location in curved lipid bilayers using oriented lipid cubic phases

Abstract: This proposal will test whether membrane proteins within lipid cubic phases preferentially sit in more or less curved bilayer regions, using our recent advances in preparing cubic phase films with high orientation, combined with 2D Grazing Incidence-SANS. This is a resubmission of previous ISIS proposal RB1710446, for which panel feedback was that it was highly rated, but evidence was requested for availability of the labelled protein OmpF. We now have an e-mail from our collaborators in Newcastle confirming that they will provide labelled and unlabelled OmpF.

Principal Investigator: Dr Adam Squires
Experimenter: Miss Eleonore Mason
Experimenter: Dr Oscar Ces
Experimenter: Dr Nicoḷ Paracini
Local Contact: Dr Sarah Rogers
Experimenter: Dr Luke Clifton
Experimenter: Dr Nick Brooks
Experimenter: Dr Arwen Tyler

DOI: 10.5286/ISIS.E.RB1720203

ISIS Experiment Number: RB1720203

Part DOI Instrument Public release date Download Link
10.5286/ISIS.E.87841279 SANS2D 20 December 2020 Download
10.5286/ISIS.E.87841304 SANS2D 05 May 2021 Download
10.5286/ISIS.E.90680306 SANS2D 27 June 2021 Download

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
Select the data format above to find out more about it.

Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Dr Adam Squires et al; (2017): Testing protein location in curved lipid bilayers using oriented lipid cubic phases, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1720203

Data is released under the CC-BY-4.0 license.



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