This is a page describing data taken during an experiment at the ISIS Neutron and Muon Source. Information about the ISIS Neutron and Muon Source can be found at https://www.isis.stfc.ac.uk.
Testing protein location in curved lipid bilayers using oriented lipid cubic phases
Abstract: This proposal will test whether membrane proteins within lipid cubic phases preferentially sit in more or less curved bilayer regions, using our recent advances in preparing cubic phase films with high orientation, combined with 2D Grazing Incidence-SANS. This is a resubmission of previous ISIS proposal RB1710446, for which panel feedback was that it was highly rated, but evidence was requested for availability of the labelled protein OmpF. We now have an e-mail from our collaborators in Newcastle confirming that they will provide labelled and unlabelled OmpF.
Principal Investigator: Dr Adam Squires
Experimenter: Miss Eleonore Mason
Experimenter: Dr Oscar Ces
Experimenter: Dr Nicoḷ Paracini
Local Contact: Dr Sarah Rogers
Experimenter: Dr Luke Clifton
Experimenter: Dr Nick Brooks
Experimenter: Dr Arwen Tyler
DOI: 10.5286/ISIS.E.RB1720203
ISIS Experiment Number: RB1720203
Part DOI | Instrument | Public release date | Download Link |
---|---|---|---|
10.5286/ISIS.E.87841279 | SANS2D | 20 December 2020 | Download |
10.5286/ISIS.E.87841304 | SANS2D | 05 May 2021 | Download |
10.5286/ISIS.E.90680306 | SANS2D | 27 June 2021 | Download |
Publisher: STFC ISIS Neutron and Muon Source
Data format: RAW/Nexus
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Data Citation
The recommended format for citing this dataset in a research
publication is as:
[author], [date], [title], [publisher],
[doi]
For Example:
Dr Adam Squires et al; (2017): Testing protein location in curved lipid bilayers using oriented lipid cubic phases, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1720203
Data is released under the CC-BY-4.0 license.