ISIS Neutron and Muon Source Data Journal

Mechanism and rates of phospholipid exchange and flip-flop under physiological conditions

Abstract: Variation in lipid composition between different biological membrane and across membranes are vital for a wide range of cellular functions. Significant differences in lipid distribution have been found between different organelles and cells, yet the mechanism and rate of phospholipid flip-flop (movement of a lipid from one leaflet to the other) and exchange (movement of a lipid from one membrane to another) are currently very difficult to measure, and estimated rates span several orders of magnitude. Labelling lipids (e.g. with fluorescent groups) significantly affects the kinetics of these processes and so it is extremely important to develop chemical-label free methods to elucidate their mechanisms and kinetics. We aim to apply a neutron contrast variation based to probe the mechanism of inter-vesicle lipid exchange and intra-vesicle flip-flop, and systematically measure their rates.

Principal Investigator: Dr Nick Brooks
Experimenter: Mr Mohit Devgan
Experimenter: Miss Jenny Liu
Experimenter: Miss Zexi Xu
Experimenter: Dr Robert Law
Experimenter: Professor John Seddon
Local Contact: Dr Sarah Rogers
Experimenter: Dr Oscar Ces
Experimenter: Dr Arwen Tyler
Experimenter: Mr Alessandro Gulotta
Experimenter: Professor Robert Law

DOI: 10.5286/ISIS.E.RB1720407

ISIS Experiment Number: RB1720407

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
Select the data format above to find out more about it.

Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Dr Nick Brooks et al; (2018): Mechanism and rates of phospholipid exchange and flip-flop under physiological conditions, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1720407

Data is released under the CC-BY-4.0 license.