ISIS Neutron and Muon Source Data Journal
This is a page describing data taken during an experiment at the ISIS Neutron and Muon Source. Information about the ISIS Neutron and Muon Source can be found at https://www.isis.stfc.ac.uk.
Measurement of the hydration of B-DNA
Abstract: Water is intimately entwined structurally with DNA. Depending on its hydration state, DNA can form several forms: A, B, C, D and Z. In addition water bound in the grooves of the double helix is essential for helix stability. The naturally forming B-form of DNA as originally defined by Crick and Watson has a helical pitch of 10 base pairs every 34 angstroms, where the bases are in a flat planar conformation perpendicular to the helical axis. This form has been the subject of extensive structural, modelling and biochemical studies, as such a large body of literature exists on this form of DNA.We wish to use wide angle neutron scattering on the NIMROD beamline at ISIS to probe the role of hydration water in DNA stability. This will be a pilot study with a synthetic construct that contains a cognate and non-cognate site for the restriction endonuclease EcoRI.
Principal Investigator: Dr David Scott
Experimenter: Mr Ryan Lithgo
Local Contact: Dr Tom Headen
Experimenter: Dr Daniel Bowron
DOI: 10.5286/ISIS.E.RB1720140
ISIS Experiment Number: RB1720140
| Part DOI | Instrument | Public release date | Download Link |
|---|---|---|---|
| 10.5286/ISIS.E.87775565 | NIMROD | 29 April 2021 | Download |
Publisher: STFC ISIS Neutron and Muon Source
Data format: RAW/Nexus
Select the data format above to find
out more about it.
Data Citation
The recommended format for citing this dataset in a research
publication is as:
[author], [date], [title], [publisher],
[doi]
For Example:
Dr David Scott et al; (2018): Measurement of the hydration of B-DNA, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1720140
Data is released under the CC-BY-4.0 license.
