ISIS Neutron and Muon Source Data Journal

Dynamics of yeast lipid membranes

Abstract: A cell membrane can contain hundreds of different lipid molecules, but our understanding of the dynamics of cell membranes is very limited. As a result of a collaboration between the ILL and ESS on the deuteration of lipids from cell cultures, we can now produce 100s of milligrams of lipid extracts from yeast and other microbial cell cultures, sufficient for neutron spectroscopy studies of their dynamics. We recently carried out the first QENS experiment on IN16B at ILL to investigate the ns dynamics of total lipid extracts from P. pastoris yeast, and found that their average molecular dynamics are considerably faster than single lipid membranes such as DMPC, and could not be observed on the timescale probed by IN16B. We now propose to continue our investigation on IRIS to probe faster dynamics that should allow a comparison to DMPC and the polyunsaturated lipid PLPC.

Principal Investigator: Dr Hanna Wacklin-Knecht
Experimenter: Mr Robin Delhom
Local Contact: Dr Victoria Garcia Sakai
Experimenter: Dr Wolfgang Knecht
Experimenter: Mr Juan Manuel Orozco Rodriguez

DOI: 10.5286/ISIS.E.RB1910325

ISIS Experiment Number: RB1910325

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
Select the data format above to find out more about it.

Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Dr Hanna Wacklin-Knecht et al; (2019): Dynamics of yeast lipid membranes, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1910325

Data is released under the CC-BY-4.0 license.