ISIS Neutron and Muon Source Data Journal

Protein nanosheets assembled structures at the air-water interfaces for cell adhesion and culture

Abstract: Cell adhesion and proliferation at the surface of liquids such as oil droplets is a surprising phenomenon as it is typically accepted in the field of bioengineering that cells require solid substrates to adhere and to exert mechanical forces required for their spreading and proliferation. Our laboratory recently reported such observations and uncovered that such phenomenon was mediated by a nanoscale (15-20 nm) mechanically strong protein film (nanosheet) assembled at the interface between the two liquids. However, little is known of the morphology and structure of such protein nanosheet and the impact of such structure on their mechanics, and in turn cell behaviour. This project aims to explore the structure of protein nanosheets assembled at analogous air-water interfaces, in situ.

Principal Investigator: Dr Ali Zarbakhsh
Experimenter: Mr Pengfei Liu
Experimenter: Miss Cardee Alexis
Experimenter: Professor Julien Gautrot
Local Contact: Dr Mario Campana

DOI: 10.5286/ISIS.E.RB1820097

ISIS Experiment Number: RB1820097

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Dr Ali Zarbakhsh et al; (2018): Protein nanosheets assembled structures at the air-water interfaces for cell adhesion and culture, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1820097

Data is released under the CC-BY-4.0 license.