ISIS Neutron and Muon Source Data Journal

Interaction of the nucleic acid, DNA, with a model biomembranes of varying rigidity at the oil-water interface

Abstract: In this experiment we aim to investigate, using neutron reflectometry in combination with contrast variation, the intermolecular interaction between the nucleic acid, calf thymus DNA monolayer comprised of a mixture of phospholipid(DSPC) with varying amounts of cholesterol at the oil-water interface in the presence of divalent cation. Of particular interest here is the conformational order of the film as function of DNA concentration which will be studied. In particular the effect of membrane rigidity at the oil/water interface has not been resolved to date and is an important aspect of the current project. The information gained in the study will be invaluable in understanding of the structure and transport properties of the cell membrane as well as helping in the development of vehicles to improve the delivery of nucleic acids (i.e. gene).

Principal Investigator: Professor Jayne Lawrence
Experimenter: Mr Pengfei Liu
Experimenter: Mr Charlie Pearce
Local Contact: Dr Maxmilian Skoda
Experimenter: Dr Richard Campbell
Experimenter: Dr Ali Zarbakhsh

DOI: 10.5286/ISIS.E.RB1820538

ISIS Experiment Number: RB1820538

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
Select the data format above to find out more about it.

Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Jayne Lawrence et al; (2019): Interaction of the nucleic acid, DNA, with a model biomembranes of varying rigidity at the oil-water interface, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1820538

Data is released under the CC-BY-4.0 license.