ISIS Neutron and Muon Source Data Journal
This is a page describing data taken during an experiment at the ISIS Neutron and Muon Source. Information about the ISIS Neutron and Muon Source can be found at https://www.isis.stfc.ac.uk.
Lipid exchange in circularized nanodiscs
Abstract: Nanodiscs (NDs) are discoidal lipid-protein aggregates composed by a lipid bilayer surrounded by a protein belt. NDs are suitable systems to investigate membrane protein structure in solution by scattering (e.g. SANS and SAXS), spectroscopy (e.g. NMR) and Cryo-EM techniques. Recently, we developed three types of protein belts (named csE3, cs1D1 and csDH5), aiming at improving ND thermal and time stability for membrane protein structure characterization in solution. Here we propose to complement the structural characterization of csE3, cs1D1 and csDH5 NDs by investigating lipid-exchange between NDs emerged as a key dynamic aspect to determine the ND capability to mimic lipid membranes.
Principal Investigator: Dr Alessandra Luchini
Experimenter: Miss Abigail Barclay
Experimenter: Mr Nicolai Johansen
Experimenter: Mr Frederik Tidemand
Local Contact: Dr Najet Mahmoudi
Experimenter: Professor Lise Arleth
DOI: 10.5286/ISIS.E.RB1910265
ISIS Experiment Number: RB1910265
| Part DOI | Instrument | Public release date | Download Link |
|---|---|---|---|
| 10.5286/ISIS.E.RB1910265-1 | SANS2D | 30 May 2022 | Download |
Publisher: STFC ISIS Neutron and Muon Source
Data format: RAW/Nexus
Select the data format above to find
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Data Citation
The recommended format for citing this dataset in a research
publication is as:
[author], [date], [title], [publisher],
[doi]
For Example:
Dr Alessandra Luchini et al; (2019): Lipid exchange in circularized nanodiscs, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1910265
Data is released under the CC-BY-4.0 license.
