ISIS Neutron and Muon Source Data Journal

Effect of End Group on RAFT-Polymers for Lipid Nanodisc Stabilisation

Abstract: Polymer stabilized lipid nanodiscs offer enormous potential as tools for enabling membrane protein structural studies & biophysics. Previously we studied discs formed using a poly(styrene-alt-maleic acid) (PSMA) copolymer, and variants of this material synthesised using RAFT controlled radical polymerisations. The RAFT method results in a C12 chain on the end of the styrene tail of the polymer, from the RAFT agent that controls the polymerisation reaction. Removal of the RAFT endgroup leaves a hydrophilic cyanoisopropyl group, which can be then further functionalised. We have prepared a series of polymers where the endgroup has converted to a cyanoisopropyl group, which, based on DLS measurements, appears to destabilise polymer aggregation in solution and favour stability of larger lipid aggregates. Here we wish to ensure these polymers form discs and probe effects on disc structure.

Principal Investigator: Professor Karen Edler
Experimenter: Dr Luke Clifton
Experimenter: Dr James Doutch
Local Contact: Dr Robert Dalgliesh
Experimenter: Mr George Neville
Experimenter: Ms Kerrie Morrison

DOI: 10.5286/ISIS.E.RB1910182

ISIS Experiment Number: RB1910182

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Karen Edler et al; (2019): Effect of End Group on RAFT-Polymers for Lipid Nanodisc Stabilisation, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1910182