ISIS Neutron and Muon Source Data Journal

Mimicking Pathogens : Floating Gram Negative Bacterial Membranes Containing the O-antigen

Abstract: Recently we have been able to show two advances in the creation of realistic models of the Gram negative bacterial outer surface for use in biophysical and structural studies. First we have incorporated smooth (O-antigen containing) lipopolysaccharides into our silicon supported membrane models. Secondly, we have improved the ease of floating bilayer production by depositing the asymmetric LPS containing membrane mimetics below a thiol-alkane self assembled monolayer. Here we wish to combine these advances to examine free floating, asymmetric, smooth LPS containing membranes and use these systems to re-examine the effect on the Gram-negative bacterial outer membrane of divalent cation sequestration.

Principal Investigator: Professor Jeremy Lakey [Deactivated] [Deactivated]
Local Contact: Dr Maxmilian Skoda
Experimenter: Dr Martynas Gavutis
Experimenter: Dr Nicolò Paracini
Local Contact: Dr Jos Cooper
Experimenter: Dr Luke Clifton

DOI: 10.5286/ISIS.E.RB1910619

ISIS Experiment Number: RB1910619

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Jeremy Lakey [Deactivated] [Deactivated] et al; (2019): Mimicking Pathogens : Floating Gram Negative Bacterial Membranes Containing the O-antigen, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1910619

Data is released under the CC-BY-4.0 license.