ISIS Neutron and Muon Source Data Journal

SANS Studies of Nanostructures of Microfluidically Fabricated lipid Nanoparticles for Plasmid DNA Delivery

Abstract: We have developed a new approach for forming lipid nanoparticles (LNP) with and without interfering RNA and DNA from a microfluidic setup. The LNPs are formed by an ionisable cationic lipid, helper lipid, cholesterol and a poly(ethylene glycol) (PEG) lipid, and the different components can self-assemble with the payload via the microfluidic mixing system. These RNA or DNA loaded LNPs mimic those reported in the literature as promising delivery vectors and work by collaborators in MedImmune has demonstrated their high transfection efficacy and low toxicity for different cell lines. Following our DLS and related characterisations, we seek to exploit the unique feature of SANS coupled with contrast variation to determine the inner structures of LNPs, thus relating the plasmid DNA transfection to the LNP inner structures tuned by different help lipids.

Principal Investigator: Professor Jian Lu
Experimenter: Dr Xuzhi Hu
Experimenter: Mr Huayang Liu
Experimenter: Dr Peixun Li
Local Contact: Dr Najet Mahmoudi
Experimenter: Dr Zongyi Li
Local Contact: Dr Steve King
Experimenter: Dr Mingrui Liao

DOI: 10.5286/ISIS.E.RB1910214

ISIS Experiment Number: RB1910214

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Jian Lu et al; (2019): SANS Studies of Nanostructures of Microfluidically Fabricated lipid Nanoparticles for Plasmid DNA Delivery, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1910214

Data is released under the CC-BY-4.0 license.