ISIS Neutron and Muon Source Data Journal

Time-resolved SANS of RNA packaging into virus capsids: Effect of RNA on the protein self-assembly pathway

Abstract: The genetic code of viruses is typically protected by a spherical protein container. This protein capsid is assembled from hundreds of individual proteins in a spontaneous self-assembly process. We currently lack a detailed structural model for how self-assembly proceeds from the isolated subunits into the final protein capsid and how RNA/DNA gets incorporated into the capsids. In this proposal, we will study the capsid assembly process of hepatitis B virus using time resolved small angle neutron Scattering. Assembly of capsid will be triggered by mixing of capsid building blocks and RNA and the reaction will be followed over time. Using solvent contrast matching the structural transitions in protein and RNA over time can be followed separately and this will give a unique insight into the capsid assembly pathway.

Principal Investigator: Dr Ingemar André
Experimenter: Dr Ryan Oliver
Experimenter: Dr Wojciech Potrzebowski
Experimenter: Dr Najet Mahmoudi
Experimenter: Dr Ryan Oliver

DOI: 10.5286/ISIS.E.RB1810397

ISIS Experiment Number: RB1810397

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Dr Ingemar André et al; (2019): Time-resolved SANS of RNA packaging into virus capsids: Effect of RNA on the protein self-assembly pathway, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1810397

Data is released under the CC-BY-4.0 license.