ISIS Neutron and Muon Source Data Journal

Investigation of the translocation potential of therapeutic macromolecules into cell-derived exosomes

Abstract: Exosomes are released by cells are responsible for cell-cell communication of macromolecules and the development of disease such as cancer. These nanoparticles are loaded with biologically active macromolecules, such interfering RNA and message RNA that mediate effects on the recipient cell. The discovery that Anthrax toxin can delivers toxins to neighbouring cells using exosome biogenesis has afforded an opportunity to develop a novel strategy for the investigation of this important pathological process, and to investigate a manufacturing process for the development of therapeutic exosomes for the treatment of common diseases using macromolecular therapies. We propose to investigate the ability of this toxin based system to package cell-derived exosomes with therapeutic macromolecules using neutron reflectometry to chart the translocation of these molecules in to the exosome.

Public release date: 12 May 2020

Principal Investigator: Dr Paul Dyer
Experimenter: Miss Rebecca Jones
Experimenter: Dr Rob Barker
Local Contact: Dr Rebecca Welbourn
Experimenter: Miss Susan Shorter

DOI: 10.5286/ISIS.E.86387589

Parent DOI: 10.5286/ISIS.E.RB1710290

ISIS Experiment Number: RB1710290

Part Number: 1

Date of Experiment: 08 May 2017

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Dr Paul Dyer et al; (2017): Investigation of the translocation potential of therapeutic macromolecules into cell-derived exosomes, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.86387589

Data is released under the CC-BY-4.0 license.