ISIS Neutron and Muon Source Data Journal
This is a page describing data taken during an experiment at the ISIS Neutron and Muon Source. Information about the ISIS Neutron and Muon Source can be found at https://www.isis.stfc.ac.uk.
Structure-function studies of Replication Protein A, a crucial player in genome stability
Abstract: Replication protein A (RPA), the eukaryotic ssDNA binding protein, is a heterotrimeric protein required for almost all aspects of cellular DNA metabolism. RPA binds single-stranded DNA with high affinity. We want to elucidate the roles of RPA in DNA replication, using integrated structural and biophysical methods, using the Archaeon Pyrococcus abyssi as a model organism. RPA is an ideal target for small angle scattering studies as there is partial structural information available on the individual protein subunits, an incomplete structure available of the trimerization core, and of partial structures of portions of RPA bound to DNA. We will carry out SANS to determine the solution structures of each RPA protein component, contrast variation SANS experiments to obtain the structure of the two possible protein dimers, the full RPA trimer structure, as well as the complexes with DNA.
Public release date: 21 March 2015
Principal Investigator: Dr Laura Spagnolo
Experimenter: Dr Cameron Neylon
Experimenter: Mr Nassos Adamopoulos
Experimenter: Mr Giuseppe Cannone
DOI: 10.5286/ISIS.E.24088041
ISIS Experiment Number: RB1120155
Part Number: 1 - SANS2D
Date of Experiment: 12 October 2011
Publisher: STFC ISIS Neutron and Muon Source
Data format: RAW/Nexus
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Data Citation
The recommended format for citing this dataset in a research
publication is as:
[author], [date], [title], [publisher],
[doi]
For Example:
Dr Laura Spagnolo et al; (2011): Structure-function studies of Replication Protein A, a crucial player in genome stability, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.24088041
