ISIS Neutron and Muon Source Data Journal

This is a page describing data taken during an experiment at the ISIS Neutron and Muon Source. Information about the ISIS Neutron and Muon Source can be found at https://www.isis.stfc.ac.uk.

ISIS Neutron and Muon Source

ISIS Neutron and Muon Data

Characterizing lipid nanocarriers (LNCs) for targeting intracellular delivery of siRNA

Data collected on the
SANS2D instrument
at the ISIS facility

Abstract: Gene therapy by RNA interference is rapidly developing as a personal field of cancer treatment. Small interfering RNA (siRNA) plays a key role, enabling the targeting of disease-causing proteins. The successful application of siRNA relies heavily on efficient delivery systems. Lipid nanocarriers (LNCs) are used as drug delivery systems. However, tissue-specific targeting remains a key challenge to overcome. In this regard, we aim to design T7 peptide-functionalized liposomes, known for their ability to bind to the human transferrin receptor (hTfR), highly expressed on both the blood-brain barrier and blood?tumor barrier for glioma. A modified T7 peptide was synthesized and the highly selective thiol-maleimide reaction was chosen for further functionalization to DOPE-PEG2000-Mal. DOPE-PEG2000-Mal-T7 will be used as an LNCs substituent, with DPPC, DOTAP and cholesterol. By SANS measurements, exploiting the contrast-matching technique by using hydrogenated and deuterated lipids as components of the LNCs, we aim to understand where DOPE-PEG2000-Mal-T7 is located and the interaction between LNCs and hTfR.

Public release date: 24 March 2028

Principal Investigator: Professor Luigi Paduano
Local Contact: Dr Najet Mahmoudi
Experimenter: Mrs Ilaria De Cristofaro
Experimenter: Mr Alessandro Cangiano
Local Contact: Dr Leide Cavalcanti
Experimenter: Dr Noemi Gallucci

DOI: 10.5286/ISIS.E.RB2510388-1

Parent DOI: 10.5286/ISIS.E.RB2510388

ISIS Experiment Number: RB2510388

Part Number: 1

This dataset is NOT yet publicly available.
The release date is: 24 March 2028

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the dataset

Date of Experiment: 18 March 2025

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Luigi Paduano et al; (2025): Characterizing lipid nanocarriers (LNCs) for targeting intracellular delivery of siRNA, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2510388-1

Data is released under the CC-BY-4.0 license.