ISIS Neutron and Muon Source Data Journal

NR Study of Helper Lipid Effect on Interaction between Lipid Nanoparticles and a Model Lipid Bilayer Mimicking Endosome Membrane

Abstract: LNP has become one of the hottest topics during the ongoing pandemic of COVID-19 due to their application as the gene delivery vector for both the Pfizer?BioNTech and Moderna mRNA vaccines. LNPs are internalised by the cells through endocytosis and the endosomal escape process releases the payloads: this process is often the major bottleneck in achieving high cell transfections. Neutron reflection is particularly powerful to study the interaction between the LNPs and a model supported lipid bilayer (SLB) mimicking an endosome membrane. The aim of this study is to determine how different lipids help mediate the interaction of LNPs with lipid membrane bilayer, mimicking an important step in endocytosis, as a function of crucial factors such as temperature and solution pH.

Public release date: 06 October 2025

Principal Investigator: Professor Jian Lu
Experimenter: Mr kangcheng shen
Experimenter: Dr Xuzhi Hu
Experimenter: Miss Tianhao Ge
Experimenter: Dr Zeyuan Zhuang
Experimenter: Dr Mingrui Liao
Local Contact: Dr Mario Campana
Experimenter: Dr Zongyi Li

DOI: 10.5286/ISIS.E.RB2220792-1

Parent DOI: 10.5286/ISIS.E.RB2220792

ISIS Experiment Number: RB2220792

Part Number: 1

Date of Experiment: 03 October 2022

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Jian Lu et al; (2022): NR Study of Helper Lipid Effect on Interaction between Lipid Nanoparticles and a Model Lipid Bilayer Mimicking Endosome Membrane, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2220792-1

Data is released under the CC-BY-4.0 license.