ISIS Neutron and Muon Source Data Journal

SANS study of pH-induced structural alteration of lipid nanoparticles for gene delivery

Abstract: We have studied lipid nanoparticles (LNPs) prepared from an ionisable cationic lipid, a helper lipid, a cholesterol and a poly(ethylene glycol) (PEG) lipid via a microfluidic system. This 4-component system mimics the LNP nanocarriers used in the delivery of COVID-19 vaccines by Pfizer and Moderna. Our recent studies have revealed vast variations in the transfection and expression efficiency of LNPs in both in vitro and in vivo experiments from substituting some ingredients with their analogs. Furthermore, pH environment also has a huge impact on their nanostructures. As LNPs are internalized via emdosomal cycling during which pH gradually becomes acidic, we request 3 days of SANS2D to undertake the proposed work and cover the widest Q range for better structural resolution,

Public release date: 25 July 2025

Principal Investigator: Professor Jian Lu
Experimenter: Miss Tianhao Ge
Experimenter: Dr Xuzhi Hu
Experimenter: Dr Zeyuan Zhuang
Experimenter: Miss Lin Zhang
Local Contact: Dr Gregory Smith
Experimenter: Dr Zongyi Li

DOI: 10.5286/ISIS.E.RB2210276-2

Parent DOI: 10.5286/ISIS.E.RB2210276

ISIS Experiment Number: RB2210276

Part Number: 2

Date of Experiment: 24 July 2022

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Jian Lu et al; (2022): SANS study of pH-induced structural alteration of lipid nanoparticles for gene delivery, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2210276-2

Data is released under the CC-BY-4.0 license.