ISIS Neutron and Muon Source Data Journal

Plant Antimicrobial Protein Interactions with Self-Assembled Floating Membrane Models of Plant and Bacterial Membranes

Abstract: Previously (RB1910140) we developed free floating model plant and pathogen membranes where complex unsaturated lipid mixtures and sterols which can be self-assembled adjacent to anionic PEGylated thiol self-assembling monolayers (SAM). Here, we will examine the interaction of plant defence protein purothionin, under physiologically accurate conditions using this sample system. Furthermore, we will examine whether this novel sample system has any particular issues relating to this kind of interaction studies and how the disruption of the floating bilayer affects the system behaviour. For our study we will generate four different membranes ? two plant plasma membrane models of different complexity in their lipid composition and two bacterial models. The distribution of purothionin across each membrane, as well as the position and condition of each membrane itself will be elucidated.

Public release date: 09 June 2024

Experimenter: Mrs Laura John
Experimenter: Professor Mark Sansom
Experimenter: Professor Gail Preston
Local Contact: Dr Luke Clifton

DOI: 10.5286/ISIS.E.RB2010152-3

Parent DOI: 10.5286/ISIS.E.RB2010152

ISIS Experiment Number: RB2010152

Part Number: 3

Date of Experiment: 28 May 2021

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
Select the data format above to find out more about it.

Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Mrs Laura John et al; (2021): Plant Antimicrobial Protein Interactions with Self-Assembled Floating Membrane Models of Plant and Bacterial Membranes, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2010152-3

Data is released under the CC-BY-4.0 license.