ISIS Neutron and Muon Source Data Journal

SANS for Structure Study of Microfluidic Lipid Nanoparticles for Plasmid DNA Delivery

Abstract: Using the newly developed approach for producing lipid nanoparticles (LNP) with and without DNA from a microfluidic setup, we propose to determine the detailed structures within the LNPs formed by an ionisable cationic lipid, helper lipid, cholesterol and a poly(ethylene glycol) (PEG) lipid, through a set of SANS runs under different isotopic contrasts. These DNA loaded LNPs mimic those reported in the literature as promising delivery vectors and work by collaborators in AstraZeneca have demonstrated their high transfection efficacy and low toxicity for different cell lines. Following our recent home lab characterisations, we seek to exploit the unique capability of SANS coupled with contrast variations to determine the inner structures of LNPs, thus relating the plasmid DNA transfection to the LNP inner structures tuned by different help lipids.

Public release date: 22 September 2022

Principal Investigator: Professor Jian Lu
Experimenter: Dr Peixun Li
Experimenter: Dr Chris van der Walle
Experimenter: Dr Najet Mahmoudi
Experimenter: Dr Zongyi Li
Local Contact: Dr Steve King

DOI: 10.5286/ISIS.E.RB1920410-1

Parent DOI: 10.5286/ISIS.E.RB1920410

ISIS Experiment Number: RB1920410

Part Number: 1

Date of Experiment: 20 September 2019

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Jian Lu et al; (2019): SANS for Structure Study of Microfluidic Lipid Nanoparticles for Plasmid DNA Delivery, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1920410-1

Data is released under the CC-BY-4.0 license.