ISIS Neutron and Muon Source Data Journal

Conformation of Single Condensed RNA Molecules in Polyethylenimine Solutions Used for Vaccination

Abstract: Messenger RNA (mRNA) constitutes a novel option for therapeutic intervention as, after application to a patient, virtually any therapeutic protein can be expressed. Currently, several RNA based products for vaccination against Covid-19 are in clinical development. For application to patients, delivery vehicles, usually consisting of cationic lipids or polymers, are required. Among the polymers, polyethylenimine (PEI), is an established system for RNA delivery. We have identified a novel form of RNA polyplex formulations based on PEI, which has demonstrated to be suitable for vaccination in combination with self-amplifying RNA (saRNA), that results in superior activity in. Here we want to further investigate the structure and molecular organization of these RNA complexes by SANS using D2O/H2O contrast variation, that can lead to further improvements in such PEI-saRNA formulations.

Public release date: 03 October 2023

Principal Investigator: Dr Heinrich Haas
Experimenter: Mr Jorge Moreno Herrero
Local Contact: Dr Leide Cavalcanti

DOI: 10.5286/ISIS.E.RB2000156-2

Parent DOI: 10.5286/ISIS.E.RB2000156

ISIS Experiment Number: RB2000156

Part Number: 2

Date of Experiment: 29 September 2020

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Dr Heinrich Haas et al; (2020): Conformation of Single Condensed RNA Molecules in Polyethylenimine Solutions Used for Vaccination, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2000156-2

Data is released under the CC-BY-4.0 license.